keywords: Antioxidant, ethanol, extract, liver, hepatoprotective, purple-onion
Carbon tetrachloride is widely used for experimental induction of liver damage by lipid peroxidation, decreased activities of antioxidant enzymes and generation of free radicals. Onions are known to contain anthocyanins and the flavonoids, quercetin and kaempferol which have free radical scavenging activity. The study was designed to evaluate the hepatoprotective effect of ethanol extract of purple onion against carbon tetrachloride-induced hepatotoxicity in Wistar rats. Liver damage was induced in experimental animals by administering CCl4 (2 ml/kg). The ethanolic extract of purple onion (200, 400 and 600 mg/kg) was given for seven days before CCl4 intoxication. Hepatoprotective effect was studied by assaying the activities of serum marker enzymes like aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and other biochemical parameters such as direct and total bilirubin, total protein and albumin and lipid profile. Antioxidant enzymes like catalase and glutathione-S transferase levels were also assayed. The results of the present investigation revealed that the given dose of Carbon tetrachloride produced significant increase in aspartate aminotransferase, alanine amino transferase, and alkaline phosphatase while there was a significant decrease in total protein level in group 2. There was also a significant decrease in the activities of CAT and GST in CCl4 treated group all indicating impaired liver function. The pre-treatment with ethanol extract of purple onion decreased the CCl4-induced elevation in liver enzymes and other biochemical parameters. These results show that ethanol extract of purple onion have potential therapeutic effect on hepatotoxicity.
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