(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170


Pages: 274-276
I. A. Bello, R. G. Ayo, I. Isyaku and I. G. Kizito

keywords: Combretum lamprocarpum, leaf, triterpenoid, lupeol, ethyl acetate, Combretaceae


The purpose of this work was to isolate and characterize the bioactive compound from the leaf of Combretum lamprocarpum Diels (Combretaceae). The powdered plant material was extracted using Microwave-Assisted Extraction technique and n-hexane, chloroform, ethyl acetate, and methanol as solvents. The ethyl acetate extract was loaded on a silica gel column using n-hexane and ethyl acetate as eluents. A pure compound was isolated and its Infra-Red, proton and carbon 13 Nuclear Magnetic Resonance spectra were measured. A pair of broad singlet protons appeared in the proton Nuclear Magnetic Resonance spectra at δ 4.54 and 4.67 (1H each). This was indicative characterization of the exo-methylene group present in the Lupane type of triterpenoids. The compound was characterized as Lupeol by comparing its carbon 13 Nuclear Magnetic Resonance spectroscopic data to the literature data. A compound, Lupeol (Lup-20(29)-en-3β-ol) was isolated from the ethyl acetate extract of the leaf of Combretum lamprocarpum. Thorough literature search shows that this compound was isolated from this plant for the first time.


Aderogba MA, Kgatle DT, McGaw LJ & Eloff JN 2012. Isolation of antioxidant constituents from Combretum apiculatum subspecies apiculatum. South African J. Botany, 79: 125-131. Adjakpa JB, Ahoton LE, Obossou FK & Ogougbé C 2016. Ethnobotanical study of Senegal custard apple (Annona senegalensis Pers.) in Dassa-Zoumétownship, Republic of Benin. Int. J. Bio. & Chem. Sci., 10(5): 2123–2137. Bisoli E, Garcez WS, Hamerski L, Tieppo C & Garcez FR 2008. Bioactive pentacyclic triterpenes from the stems of Combretum laxum. Molecules, 13(11): 2717-2728. Eloff JN, Katerere DR & McGaw LJ 2008. The biological activity and chemistry of the southern African combretaceae. Journal of Ethnopharmacology, 119(3): 686-699. Fernandes FFA, Tomaz MA, El-Kik CZ, Monteiro-Machado M, Strauch MA, Cons BL, Tavares-Hanriques MS, Cintra AC, Facundo VA & Melo PA 2014. Counteraction of Bothrops snake venoms by Combretum leprosum root extract and arjunolic acid. Journal of Ethnopharmac., 155(1): 552–562. http://doi.org/10.1016/j.jep.2014.05.056 Fyhrquist P, Mwasumbi L, Vuorela P, Vuorela H, Hiltunen R, Murphy C & Adlercreutz H 2006. Preliminary antiproliferative effects of some species of Terminalia, Combretum and Pteleopsis collected in Tanzania on some human cancer cell lines. Fitoterapia, 77(5): 358-366. Gallo MBC & Sarachine MJ 2009. Biological Activities of Lupeol. International journal of biomedical and pharmaceutical sciences, 3(1): 46-66. Gibreel HH, Kordofani MAY, Warrag EI & Ahmed HO 2013. Medicinal value and ecotaxonomy of the flora of Blue Nile State-Sudan. J. Chem. & Pharmac. Res., 5(2): 36-43. Mbwambo ZH, Mushi NF, Nondo RS, Kidukuli AW & Mwangomo DT 2013. Antibacterial, antioxidant, cytotoxic activities and preliminary phytochemical screening of extracts from Combretum schumannii Engl. J. Med. Plants Res., 7(33): 2483-2488. Offiah NV, Makama S, Elisha IL, Makoshi MS, Gotep JG, Dawurung CJ, Oladipo OO, Elisha IL, Samuel AL & Shamaki D 2012. Survey of herbal remedies used by Fulani herdsmen in the management of animal diarrhoea in Plateau State, Nigeria. J. Med. Plants Res., 6(31): 4625–4632. Petronella A, Pannitteri G & Testa U 2009. Triterpenoids as new promising anticancer drugs. Anti-Cancer Drugs, 20(10): 880-892. Roy R, Singh RK, Jash SK, Sarkar A & Gorai D 2014. Combretum quadrangulare (Combretaceae): Phytochemical Constituents and Biological activity. Indo-American J. Pharmac. Res., 4(8): 3416-3430. Saleem M 2009. Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Letters, 285(2): 109–115. http://doi.org/10.1016/j.canlet.2009.04.033 Sánchez-burgos JA, Ramírez-mares MV, Gallegos-infante JA & González-laredo RF 2015. Isolation of lupeol from white oak leaves and its antiinflammatory activity. Industrial Crops and Products, 77(1): 827–832. Schwikkard S, Zhou BN, Glass TE, Sharp, JL, Mattern MR, Johnson RK & Kingston DG 2000. Bioactive compounds from Combretum erythrophyllum. Journal of Natural Products, 63(7): 1046. Wang LQ, Wu MM, Liu JP, Li Y, Hua Y, Wang YY, Li XY, Chen YG & Wang JH 2011. Five new diarylpropan-1-ols from Combretum yunnanense. Planta Medica, 77(16): 1841-1844. Yahaya O, Yabefa JA & Usman B 2012. Phytochemical Screening and Antibacterial Activity of "Combretum glutinosum" Extract against Some Human Pathogens. British J. Pharmacol. & Toxicol., 3(5): 233-236.